SUSPENDED: SARS-CoV-2 - ISIDORe project

Our cell models are available for the following types of analysis:

Virus quantification – infectious particles (TCID₅₀, Plaque assay)
Virus quantification – non-infectious particles (qRT-PCR)
Neutralization
Cell viability/cytoxicity
Immunological assays (ELISA, Flow cytometry)
Bioimaging (Immunofluorescence, electron microscopy, confocal microscopy, histology, etc.)

To make sure that the type of analysis you are interested in can be performed in specific cell models, please do not hesitate to contact us exvivo-invivo-services@isidore-project.eu

Organoids

Human
Lung
Nose
Blood vessel
Liver
Kidney
Brain
3D tissue and cell printer
Animal
Lung	Cattle
Intestinal	Cattle, Chicken

Table 1: Organoids

Air Liquid Interface (ALI) polarised cells

Human
Lung
Airway nasal
Airway tracheal
Airway bronchial
CALU-3
A549
RPMI-2650
Animal
Lung	Cattle, Chicken, Pig
Airway nasal	Sheep, Pig
Airway tracheal	Sheep, Pig, Mouse
Airway bronchial	Sheep, Pig
Intestinal	Cattle, Chicken

Table 2: Air Liquid Interface (ALI) polarised cells

Primary cells

Human
Immune cells ( macrophages, dendritic cells)
Lung
Cultures from surgical specimens
Animal
Immune cells (monocytes, macrophages, lymphocytes, dendritic cells)	Cattle, Sheep, Pig
Lung	Cattle, Sheep, Bat
Bronchial	Pig
Intestine	Bat

Table 3: Primary cells

Tissue section cultures

Human
Placental precision-cut slices
Animal
Lung Precision Cut slices	Mouse, Hamster, Pig, Sheep, Cattle,
Liver Precision Cut slices	Sheep

Table 4: Tissue section cultures

Organotypic cultures

Animal
Lung	Chicken, Ferret, Pig
Trachea	Chicken, Ferret, Pig

Table 5: Organotypic cultures

Immortalised cell lines

Human
CALU-3
A549
CACO-2
HEP-2
HUH-7
HTR-8
RPMI-2650
HEPA-RG
Nasal cell line
Animal
VERO E6, VERO CCL81	African Green Monkey
MDCK	Dog
Lung	Chicken
Bronchial	Pig

Table 6: Immortalized cell lines

Stem cells

Human
Mesenchymal
Redifferentiated IPSCS (Induced pluripotent stem cells)
Animal
Mesenchymal	Cattle, Sheep, Pig, Mink, Rabbit

Table 7: Stem cells

Engineered cells

Animal
VERO HSLAM ((Human) signaling lymphocytic activation molecule)	African Green Monkey
VERO E6 with fluorescent viability reporter gene (m-Cherry-H2B (Histone-2B) Protein)	African Green Monkey

Table 8: Engineered cells

Other

Custom cell requests may be considered.

Human
Preparation of tissues
Swab/saliva collection devices
Animal
Preparation of tissues	Mammalian and Avian tissues
Preparation of little organs for clearing (Lungs) with I and U DISCO	Mouse

Table 9: Other cells and services