Respiratory pathogens - ISIDORe project

Respiratory pathogens (non-SARS-CoV-2)
Coronaviridae, Orthomyxoviridae, etc.

Our cell models are available for the following types of analysis:

Virus quantification – infectious particles (TCID₅₀, Plaque assay)
Virus quantification – non-infectious particles (qRT-PCR)
Neutralization
Cell viability/cytoxicity
Immunological assays (ELISA, Flow cytometry)
Bioimaging (Immunofluorescence, electron microscopy, confocal microscopy, histology, etc.)

To make sure that the type of analysis you are interested in can be performed in specific cell models, please do not hesitate to contact us exvivo-invivo-services@isidore-project.eu

Organoids

Human
Lung
Nose
Blood vessel
Liver
Kidney
Brain
3D tissue and cell printer
Animal
Lung	Pig

Table 1: Organoids

Air Liquid Interface (ALI) polarised cells

Human
Lung
Airway nasal
Airway tracheal
Airway bronchial
CALU-3
A549
Animal
Airway nasal	Sheep
Airway tracheal	Sheep, Mouse
Airway bronchial	Sheep

Table 2: Air Liquid Interface (ALI) polarised cells

Primary cells

Human
Immune cells (macrophages, dendritic cells)
Animal
Immune cells (monocytes, macrophages, lymphocytes, dendritic cells)	Cattle, Sheep, Pig
Bronchial	Pig
Lung	Bat
Intestine	Bat
Kidney (Avian infectious bronchitis virus model)	Chicken
Embryo Kidney (Avian Influenza model)	Chicken

Table 3: Primary cells

Tissue section cultures

Human
Lung precision-cut slices
Placental precision-cut slices
Animal
Lung precision-cut slices	Mouse, Hamster, Pig, Sheep, Cattle, Chicken

Table 4: Tissue section cultures

Immortalised cell lines

Human
CALU-3
A549
CACO-2
HEP-2
HUH-7
HTR-8
Animal
VERO E6, VERO CCL81	African Green Monkey
MDCK	Dog
DF1 Embryonal fibroblasts	Chicken

Table 6: Immortalized cell lines

Stem cells

Human
Mesenchymal
Animal
Mesenchymal	Cattle, Sheep, Pig, Mink, Rabbit

Table 7: Stem cells

Engineered cells

Animal
VERO HSLAM ((Human) signaling lymphocytic activation molecule)	African Green Monkey

Table 8: Engineered cells

Other

Custom cell requests may be considered.

Human
Preparation of tissues
Swab/saliva collection devices
Animal
Embryonated Specific Pathogen Free Eggs (Avian Influenza model)	Chicken
Preparation of tissues	Mammalian and Avian tissues

Table 9: Other cells and services

Apply for a cell models service: Respiratory pathogens

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