DETECTION & CHARACTERIZATION OF IMMUNE TISSUES & CELLS
Provided to you by: EATRIS, VetBioNet, Infrafrontier & ERINHA
Contact
Patricia Carvajal-Vallejos
patriciacarvajal@eatris.eu
- Immune-function profiling
This service is available for access regardless of the call for proposals and research topic that you select in your pre-application form. Antigen-specific T proliferation and cytokine, chemokine and immune factor analysis (mass cytometry, FACSCanto, LSR IV, Symphony, Helios, Bio-Plex Multiplex immunoassay). - Immune-cell profiling
This service is available for access in the following calls for proposals: CALL I (SARS-CoV-2), CALL IV (non-SARS-CoV-2 respiratory pathogens), CALL V (vector-borne pathogens), and CALL VI (Other epidemic-prone pathogens). - Antiviral screening & mucosal immunology
This service is available for access for SARS-CoV-2 research projects. Including human airway models for testing drugs and for virus – cells interaction, to evaluate the role of individual immune subsets in different contexts against infectious disease and to characterize viral isolates. - Detection and quantification of SARS-CoV-2-specific T cells at the single cell level
This service is available for access for SARS-CoV-2 and MPXV research projects - T-cell cloning and expansion
This service is available for access for SARS-CoV-2 research projects. Determination of specificity - Detection and quantification of SARS-CoV-2-specific B cells at the single cell level
This service is available for access for SARS-CoV-2 research projects. - Access to organoids, spheroids & multifluidic devices for immune surveillance (IncuCyte live-cell imaging of spheroids)
This service is available for access for SARS-CoV-2 research projects.- Virus sequencing, non-coding RNA expression and antibody responses to viral antigens with high-content imaging and analysis.
- Access to Multi-site Immuno-analysis of SARS-CoV-2, including analysis of infected samples based on metal tag detection by mass cytometry
DETECTION & CHARACTERIZATION OF THE IMMUNE RESPONSE
Provided to you by: EATRIS, VetBioNet & ERINHA
Contact
Patricia Carvajal-Vallejos
patriciacarvajal@eatris.eu
- Medium‑ to large-scale analysis of immune proteins
The services listed below are available for access for SARS-CoV-2 research projects.- Protein/peptide identification
- Antibody Characterization / Multi-Attribute Method (MAM)
- Post-Translational Modifications Analysis (Glycosylation; Oxidation/Deamination, Glycan Identity)
- High-throughput Quantitative Proteomics – SWATH analysis (untargeted; relative quantification)
- Intact Protein Measurement
- Aggregation and refolding (SEC chromatography)
- Antibody profiling to evaluate the host immune response
- ICS-T-cell-responses (TRM), Virus-immunome profiling
Quantification of cytokines/chemokines (Bio-plex Multiplex Immunoassay), surface and intracellular detection of markers/cytokines (flow cytometry) in infected biological samples - Functional studies of pathogenicity of genetic variants at molecular and cellular level
Including virus sequencing, non-coding RNAs expression and ELISA assay, drugs; drug testing - Epigenetics of immune cells
To study genome-wide epigenetic changes including DNA methylation, histone modifications and non-coding RNAs expression - Immunoprofiling in swine and poultry
Polychromatic flow cytometry assays, single cell immunomics, MHC haplotyping, Luminex cytokine profiling
DETECTION, CHARACTERIZATION & MODULATION OF IMMUNE GENE EXPRESSION
Provided to you by: EATRIS & VetBioNet
Contact
exvivo-invivo-services@isidore-project.eu
The services listed below are available for access regardless of the call for proposals and research topic that you select in your pre-application form.
- siRNA libraries
- Human
- Swine
- Poultry
- sgRNA/CRISPR Cas9 knockout libraries
- Human
- Swine
- Poultry
- Cattle
- RNAseq transcriptomics, 16S NGS microbiome analysis, metabolomics (LC-MS) in livestock
- Swine
- Poultry
- Specific primer sets and protocols for medium-throughput Fluidigm qPCR targeting up to 96 immune genes
- Mice
- Swine
- Cattle
- Chicken
BIO IMAGING FOR IMMUNO PROFILING
Provided to you by: Euro-BioImaging
Contact
info@eurobioimaging.eu
All the services listed below are available for access at BSL-1 or BSL-2 regardless of the call for proposals & topic you select.
- Development and validation of imaging probes
Targeting of specific molecular inflammation markers or subpopulations of immune cells in surrogate infection models in vivo - Biomedical imaging technologies for monitoring the immune system
- PET
- MRI
- CT
- US
- Optical imaging
- Multimodal imaging
- High-throughput Imaging & Bioimage Analysis
PerkinElmer Opera Phenix & Molecular Devices ImageXpress Nano. Automation for sample processing and Imaging in both +37 C and RT. Image analysis pipelines and customized solutions for DNN based segmentation & classification in 2D and 3D.