A stylized globe graphic, with the EU area emphasized
ISIDORe project home / Services / Immuno-monitoring & profiling

IMMUNO MONITORING & PROFILING

State-of-the-art services for deciphering the immune response.

DETECTION & CHARACTERIZATION OF IMMUNE TISSUES & CELLS

Provided to you by: EATRIS, VetBioNet, Infrafrontier & ERINHA

Contact
David Morrow
davidmorrow@eatris.eu

  • Systems level characterization of immune cells in tissues including T cell culture
    Antigen-specific proliferation and cytokine, chemokine and immune factor analysis (mass cytometry, FACSCanto, LSR IV, Symphony, Helios)
  • Identification and isolation of tissue immunological subsets
    For deep profiling of immune cell subsets and on the analysis of WGS/WES data of patients including high-throughput multiplex immunoassays , TCR and BCR profiling and Bio-Plex multiplex cytokine (serum, plasma) analysis
  • Access to tissue explant models
    Including human airway models for testing drugs and for virus – cells interaction, to evaluate the role of individual immune subsets in different context against infectious disease and to characterize viral isolates
  • Detection and quantification of SARS-CoV-2-specific T cells at the single cell level
  • T-cell cloning and expansion
    Determination of specificity
  • Detection and quantification of SARS-CoV-2-specific B cells at the single cell level
  • Access to organoids, spheroids & multifluidic devices for immune surveillance (IncuCyte live-cell imaging of spheroids)
  • Multi-site Mass Cytometric Immuno-analysis
    Access to Multi-site Immuno-analysis of SARS-CoV-2, including analysis of infected samples based on metal tag detection by mass cytometry

DETECTION & CHARACTERIZATION OF THE IMMUNE RESPONSE

Provided to you by: EATRIS, VetBioNet & ERINHA

Contact
David Morrow
davidmorrow@eatris.eu

  • Medium‑ to large-scale analysis of the immune proteins
    • Protein/peptide identification
    • Antibody Characterization / Multi-Attribute Method (MAM)
    • Post-Translational Modifications Analysis (Glycosylation; Oxidation/Deamination, Glycan Identity)
    • High-throughput Quantitative Proteomics – SWATH analysis (untargeted; relative quantification)
    • Intact Protein Measurement
    • Aggregation and refolding (SEC chromatography)
  • Antibody profiling to evaluate the host immune response
  • Virus-specific immune responses
    Quantification of cytokines/chemokines, surface and intracellular detection of markers/cytokines (flow cytometry) in infected biological samples
  • Functional studies of pathogenicity of genetic variants at molecular and cellular level
    Including virus sequencing, non-coding RNAs expression and ELISA assay, drugs; drug testing
  • Virus neutralization testing for human and animal samples
  • Epigenetics of immune cells
    To study genome-wide epigenetic changes including DNA methylation, histone modifications and non-coding RNAs expression
  • Immunoprofiling in swine and poultry
    Polychromatic flow cytometry assays, single cell immunomics, MHC haplotyping, Luminex cytokine profiling

DETECTION, CHARACTERIZATION & MODULATION OF IMMUNE GENE EXPRESSION

Provided to you by: VetBioNet

Contact
Sascha Trapp
sascha.trapp@inrae.fr

Marie-Isabelle Thoulouze
marie-isabelle.thoulouze@envt.fr

  • siRNA libraries
    • Human
    • Swine
    • Poultry
  • sgRNA/CRISPR Cas9 knockout libraries
    • Human
    • Swine
    • Poultry
    • Cattle
  • RNAseq transcriptomics, 16S NGS microbiome analysis, metabolomics (LC-MS) in livestock
    • Swine
    • Poultry
  • Specific primer sets and protocols for medium-throughput Fluidigm qPCR targeting up to 96 immune genes
    • Mice
    • Swine
    • Cattle
    • Chicken

BIO IMAGING FOR IMMUNO PROFILING

Provided to you by: Euro BioImaging ERIC

Contact
Alessandra Viale
info@eurobioimaging.eu

  • Development and validation of imaging probes
    Targeting of specific molecular inflammation markers or subpopulations of immune cells in surrogate infection models in vivo
  • Biomedical imaging technologies for monitoring the immune system
    • PET
    • MRI
    • CT
    • US
    • Optical imaging
    • Multimodal imaging