SUSPENDED: Vector-borne pathogens

Our cell models are available for the following types of analysis:

Virus quantification – infectious particles (TCID₅₀, Plaque assay)
Virus quantification – non-infectious particles (qRT-PCR)
Neutralization
Cell viability/cytoxicity
Immunological assays (ELISA, Flow cytometry)
Bioimaging (Immunofluorescence, electron microscopy, confocal microscopy, histology, etc.)

To make sure that the type of analysis you are interested in can be performed in specific cell models, please do not hesitate to contact us exvivo-invivo-services@isidore-project.eu

Organoids

Human
Lung
Nose
Blood vessel
Liver
Kidney
Brain
Animal
Lung	Cattle, Sheep, Pig

Table 1: Organoids

Air Liquid Interface (ALI) polarised cells

Human
Lung
Airway nasal
Airway tracheal
Airway bronchial
CALU-3
A549
Animal
Lung	Cattle, Chicken
Airway nasal	Sheep
Airway tracheal	Sheep, Mouse
Airway bronchial	Sheep

Table 2: Air Liquid Interface (ALI) polarised cells

Primary cells

Human
Immune cells ( macrophages, dendritic cells)
Cultures from surgical specimens (Rift valley fever virus model)
Animal
Immune cells (monocytes, macrophages, lymphocytes, dendritic cells)	Cattle, Sheep, Pig
Lung	Cattle, Bat
Bronchial	Pig
Intestine	Bat
Hepatocytes	Sheep
Maternal epithelial cells	Sheep
Umbilical cord derived endothelial cells	Sheep
Kidney	Chicken

Table 3: Primary cells

Tissue section cultures

Human
Lung precision-cut slices
Placental precision-cut slices
Animal
Lung precision-cut slices	Mouse, Hamster, Pig, Sheep, Cattle, Chicken
Liver precision-cut slices	Sheep

Table 4: Tissue section cultures

Immortalised cell lines

Human
CALU-3
A549
CACO-2
HEP-2
HUH-7
HTR-8
BeWO
HEPA-RG (Rift Valley fever virus model)
Animal
VERO E6, VERO CCL81	African Green Monkey
MDCK	Dog
Lung	Chicken
UMNSAH/DF-1	Chicken
Bronchial	Pig
Neuro-2a	Mouse
C6/36	Aedes albopictus
Aag2	Aedes aegypti

Table 5: Immortalized cell lines

Stem cells

Human
Mesenchymal
Animal
Mesenchymal	Sheep

Table 6: Stem cells

Other

Custom cell requests may be considered.

Human
Neuronal/Glial cells
Preparation of tissues
Swab/saliva collection devices
3D-vessel-on-a-chip
Animal
Transplacental model (maternal epithelial cells and trophoblasts)	Sheep
3D-vessel-on-a-chip
Preparation of tissues	Mammalian and Avian tissues

Table 9: Other cells and services