Cell model – immortalised cell lines
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Contact
exvivo-invivo-services@isidore-project.eu
Vero E6
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability (incl. high content imaging)
- Bioimaging/Microscopy (incl. high content imaging)
- Flow cytometry
- Immunohistochemistry
- Histological methods
Vero CCL81
- Virus isolation
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR (E, RdRP, N, S)
- Omics – spatial transcriptomics
- Sequencing – NGS
- ELISA (N)
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
- Flow cytometry
- Luminex assay for antibody detection
- Histopathology
- Immunohistochemistry
- Histological methods
Calu 3
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
- Flow cytometry
- Immunohistochemistry
- Histological methods
A549
- Virus titration
- Neutralization assay
- Cytotoxicity
- Antiviral assay
- CPE (TCID50)
- qRT-PCR
- Immunofluorescence
- Cell viability (incl. high content imaging)
- Bioimaging/Microscopy ((conventional – high resolution confocal microscopy ; high content imaging)
Caco-2
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
MDCK
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
Hep-2
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
Huh-7
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
RPMI-2650
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
human nasal cell line
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
Chicken lung epithelial cells
- Virus titration (Plaque assay titration on MDCK cells)
- Cytotoxicity (CellTiter-Glo assay, automated cell numeration)
- Antiviral assay (ViralTox-Glo assay in microtiter plate format)
- qRT-PCR (Fluidigm medium throughput qPCR – primer sets for chicken innate immune genes)
- Biomaging/microscopy (CellTiter-Glo assay, automated cell numeration)
Pig bronchial epithelial cells
- Virus titration (Plaque assay titration on MDCK cells)
- Cytotoxicity (CellTiter-Glo assay, automated cell numeration)
- Antiviral assay (ViralTox-Glo assay in microtiter plate format)
- qRT-PCR (Fluidigm medium throughput qPCR)
- Biomaging/microscopy (CellTiter-Glo assay, automated cell numeration)
Cell model – primary cells
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Contact
exvivo-invivo-services@isidore-project.eu
pig macrophages
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
pig monocytes
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
pig lymphocytes
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
sheep monocytes
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
sheep macrophages (monocyte-derived)
- Virus titration
- Neutralization assay
- Antiviral assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
sheep macrophages (alveolar)
- Virus titration
- Neutralization assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
sheep macrosheep lymphocytes
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
human primary epithelial lung cells (monolayers/polarised 2D culture)
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
bovine primary epithelial lung cells (monolayers/polarised 2D culture)
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
ovine primary epithelial lung cells (monolayers/polarised 2D culture)
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
cattle macrophages (monocyte-derived)
- Virus titration
- Neutralization assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
cattle macrophages (alveolar)
- Virus titration
- Neutralization assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
primary human cell cultures from surgical specimens
- Virus isolation
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
Bat lung epithelial cells
- Virus isolation
- Virus titration
- Antiviral assay
- CPE (TCID50)
- qRT-PCR (E, RdRP, N, S)
- Sequencing – NGS
- ELISA (N)
- Immunofluorescence
- Luminex assay for antibody detection
- Histopathology
Bat intestine epithelial cells
- Virus isolation
- Virus titration
- Antiviral assay
- CPE (TCID50)
- qRT-PCR (E, RdRP, N, S)
- Sequencing – NGS
- ELISA (N)
- Immunofluorescence
- Luminex assay for antibody detection
- Histopathology
Swine bronchial epithelial cells
- Virus titration (Plaque assay titration on MDCK cells)
- Cytotoxicity (CellTiter-Glo assay, automated cell numeration)
- Antiviral assay (ViralTox-Glo assay in microtiter plate format)
- qRT-PCR (Fluidigm medium throughput qPCR )
- Biomaging/microscopy (CellTiter-Glo assay, automated cell numeration)
Cell model – mesenchymal stem cells
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Contact
exvivo-invivo-services@isidore-project.eu
rabbit mesenchymal stem cells
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
mink mesenchymal stem cells
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
bovine mesenchymal stem cells
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
pig mesenchymal stem cells
- Neutralization assay
- Antiviral assay
- PRNT/microneutralization
Cell model – Well differentiated and/or polarised cell systems
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Contact
exvivo-invivo-services@isidore-project.eu
ALI-differentiated 2D culture of primary human lung cells
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
ALI-differentiated 2D culture of primary bovine lung cells
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
Calu-3 differentiated in ALI conditions
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
A549 differentiated in ALI conditions
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
Chicken lung epithelial cells – ALI
- Virus titration (Plaque assay titration on MDCK cells)
- Cytotoxicity (CellTiter-Glo assay, automated cell numeration)
- Antiviral assay (ViralTox-Glo assay in microtiter plate format)
- qRT-PCR (Fluidigm medium throughput qPCR – primer sets for chicken innate immune genes)
- Biomaging/microscopy (CellTiter-Glo assay, automated cell numeration)
Pig bronchial epithelial cells – ALI
- Virus titration (Plaque assay titration on MDCK cells)
- Cytotoxicity (CellTiter-Glo assay, automated cell numeration)
- Antiviral assay (ViralTox-Glo assay in microtiter plate format)
- qRT-PCR (Fluidigm medium throughput qPCR)
- Biomaging/microscopy (CellTiter-Glo assay, automated cell numeration)
RPMI-2650 in ALI 2D cultures
- Vi
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
Non-conventional screening models for toxicity and ecotoxicity
Non-conventional screening models for toxicity and ecotoxicity assays:
- zebrafish eggs: up to 48h, 96 well format
- invertebrates (rotifers and artemia): 96 well format
- microalgae
Cell model – Engineered cell lines and primary cells
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Contact
exvivo-invivo-services@isidore-project.eu
Vero hSLAM ((human) signaling lymphocytic activation molecule)
- Virus isolation
- Virus titration
- Neutralization assay
- CPE (TCID50)
- qRT-PCR
- Sequencing
- ELISA
- Histopathology
- Immunohistochemistry
- In situ hybridization
Vero E6 with fluorescent viability reporter gene (m-cherry-H2B (histon-2B) protein)
- Cytotoxicity (combined with high-content imaging)
- Cell viability (high-content imaging)
- Bioimaging/Microscopy (high-content imaging)
redifferentiated iPSCs (induced Pluripotent Stem Cells)
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
Cell model – Other 2D cell model
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Contact
exvivo-invivo-services@isidore-project.eu
Preparation of mammalian tissues
- Cloning (Cell sorting on plates – 96, 384…)
- Immunofluorescence (conventional and confocal microscopy)
- Bioimaging / microscopy (conventional and confocal microscopy)
- Flow cytometry (multicolor flow cytometry, High Speed cell sorting)
- Histopathology (histology / immunochemistry)
Preparation of avian tissues
- Cloning (Cell sorting on plates – 96, 384…)
- Immunofluorescence (conventional and confocal microscopy)
- Bioimaging / microscopy (conventional and confocal microscopy)
- Flow cytometry (multicolor flow cytometry, High Speed cell sorting)
- Histopathology (histology / immunochemistry)
Swab / saliva collection devices
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
Cell model – Organoids
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Contact
exvivo-invivo-services@isidore-project.eu
human lung organoids
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
bovine lung organoids
- Cytotoxicity
- Antiviral assay
- qRT-PCR
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy (conventional – high resolution confocal microscopy)
3D tissue and cell printer
- Virus titration
- Neutralization assay
- Typing
- Cytotoxicity
- Antiviral assay
- Electron light microscopy
- Focus Forming assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
- Omics – spatial transcriptomics
- Sequencing
- Immunofluorescence
- Cell viability
- Bioimaging/Microscopy
- Flow cytometry
- Immunohistochemistry
- Histological methods
Cell model – Precision-cut tissue slices cultures
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Contact
exvivo-invivo-services@isidore-project.eu
Bovine lung PCLS (Precision Cut Lung Slices)
- qRT-PCR (Fluidigm medium-throughput qPCR – primer sets for >200 bovine genes, mainly related to immunity)
- ELISA (IL-8; IL-1b,…..)
- Immunofluorescence (confocal microscopy)
- Cell viability (LDH release assay)
Hamster PC lung tissue
- Virus isolation
- Virus titration
- Neutralization assay
- Typing
- Omics – transcriptomic analysis by nanostring assay
- Sequencing – NGS
- Histopathology (incl. Immunohistology)
Sheep primary lung PCLS
- Virus titration
- Neutralization assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
Cattle primary lung PCLS
- Virus titration
- Neutralization assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
Mouse primary lung PCLS
- Virus titration
- Neutralization assay
- CPE (TCID50)
- PRNT/microneutralization
- qRT-PCR
Cell model – Organotypic culture
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Contact
exvivo-invivo-services@isidore-project.eu
Chicken tracheal culture
- Virus isolation
- Virus titration
- CPE (TCID50)
- qRT-PCR
- Sequencing
- ELISA
Chicken lung culture
- Virus isolation
- Virus titration
- CPE (TCID50)
- qRT-PCR
- Sequencing
- ELISA
Ferret tracheal culture
- Virus isolation
- Virus titration
- CPE (TCID50)
- qRT-PCR
- Sequencing
- ELISA
Ferret lung culture
- Virus isolation
- Virus titration
- CPE (TCID50)
- qRT-PCR
- Sequencing
- ELISA
Pig tracheal culture
- Virus isolation
- Virus titration
- CPE (TCID50)
- qRT-PCR
- Sequencing
- ELISA
Pig lung culture
- Virus isolation
- Virus titration
- CPE (TCID50)
- qRT-PCR
- Sequencing
- ELISA
Cell model – Other 3D cell models
Preparation of little organs for clearing (lung of mouse) with i and u DISCO
- Cloning (Cell sorting on plates – 96, 384…)
- Immunofluorescence (conventional and confocal microscopy)
- Bioimaging / microscopy (conventional and confocal microscopy)
- Flow cytometry (multicolor flow cytometry, High Speed cell sorting)
- Histopathology (histology / immunochemistry)